Proteomic analysis of the secretome of human bone marrow-derived MSCs

MERLIN team at the Universitá degli Studi di Padova publish “Proteomic analysis of the secretome of human bone marrow-derived mesenchymal stem cells primed by pro-inflammatory cytokines” in the Journal of Proteomics, 21July 2017

Congratulations to all authors: Elisa Maffioli, Simona Nonnis, Roberta Angioni, Fabiana Santagata, Bianca Calí, Lucia Zanotti, Armando Negri, Antonella Viola, Gabriella Tedeschi.

In summary, the secretion of a broad range of bioactive molecules is believed to be the main mechanism by which mesenchymal stem cells (MSC) exert specific therapeutic effects. MSC are very versatile and respond to specific environments by producing and releasing a variety of effector molecules. To the best of our knowledge this is the first study aimed at describing the secretome of human MSC primed using a mixture of cytokines, to mimic pro-inflammatory conditions encountered in vivo, by a quantitative high-resolution mass spectrometry based approach. The main output of the study concerns the identification of a list of specific proteins involved in inflammation and angiogenesis which are overrepresented in stimulated MSC secretome.
This study found that pro-inflammatory cytokines have a strong impact on the secretome of human bone marrow-derived MSC and that the large majority of cytokine-induced proteins are involved in inflammation and/or angiogenesis.
Overall, these studies represent key steps necessary to characterize the different biology of MSC in the two organisms and design successful pre- clinical experiments as well as clinical trials. The full publication is available at: https://doi.org/10.1016/j.jprot.2017.07.012